The pTargeT(TM) Mammalian Expression Vector System is a convinient system for cloning PCR products and for expression of cloned PCR products in Mammalian cells. The vector is prepared by digestion with EcoRV followed by addition of a 3\' terminal thymidine to each end. These single 3\'-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid in two ways. First, the overhangs prevent recirculization of thge vector; second, they provide a compatible overhang for PCR products generated by vertain thermostable polymerases. These polymerases often add single deoxyadenosine, in a template-independent fashion, to be 3\'-ends of amplified fragments. The pTargeT(TM) Vector also contains a modified version of the coding sequence of the a-peptide of ß-galactosidase, which also allows recobinants to be selected using blue/white screening.
Simple PCR Cloning:\"T\" overhangs permit direct ligation of PCR products generated by thermostable enzymes such as TaqDNA polymerase.
Strong, Constitutive Expression: The CMV enhancer/promoter region allows strong, constitutive expression in many cell types.
Blue/White Screening: Allows the easy identification of recobinant clones. A single digest removes the insert DNA.
Stable Transfectants: Select from stable transfectants using the neomycin phosphotransferase gene.