
The pGEM(R)-T Easy Vector Systems are convinient systems for the cloning of PCR products. They offer all the advantages of the pGEM(R)-T Vector Systems with the added convinience of recognition sites for EcoRI and Noti flanking the insertion site. Thus several options for removal of the desired insert DNA with a single restriction digestion are provided. The pGEM(R)-T Easy Vector Systems II contains JM109 competent cells in addition to all of the pGEM(R)-T Easy Vector Systems I components.

Features:
- Flexible: The multiple cloning site is flanked by restriction enzyme sites for BstZI, NotI and EcoRI, allowing three options for removal of the insert with a single digest.
- Rapid Litgation: The 2X Rapid Litigation Buffer provided allows reactions to be completed in 1 hour at room temperature.
- Blue/White Screening: T7 and SP6 RNA polymerase promoters flank a multiple cloning region within the a-peptide coding region for ß-galactosidase. Insertional inactivation of the a-peptide allows recombinant clones to directly identified by color screening or indicator plates.
- f1 Origin of Replication: Allows the preparation of single-stranded DNA

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