Description: The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step. The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).
• Improved Productivity: Obtain genomic DNA approximately 20 minutes
• High Yield: Purify 20–30μg of DNA per prep from 1.2cm mouse tail.
• Format Choice: Perform purification by either spin or vacuum formats.