Wheat Germ Extract Plus is a highly efficient, single-tube protein expression system designed to give yields in the range of 10–80μg/ml. The extract contains all the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, and initiation, elongation and termination factors). Potassium acetate is also included at a concentration to enhance translation for a wide range of mRNAs. Protein synthesis is initiated by addition of the appropriate mRNA template, and the reaction is incubated for 2 hours at 25°C. Synthesized proteins can be analyzed by SDS-polyacrylamide gel electrophoresis or used directly in numerous applications. There is no requirement for dialysis or specific incubation equipment.

The pF3A WG (BYDV) and pF3K WG (BYDV) Flexi® Vectors are designed for in vitro expression in wheat germ extracts and are available for use with Wheat Germ Extract Plus. These vectors contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest. The BYDV elements interact with each other, form a closed loop and act synergistically to stimulate translation in wheat germ extracts, bypassing mRNA cap and polyadenylation dependencies. See the Flexi ® Vector Systems Technical Manual #TM254 for more information on the pF3A WG (BYDV) and pF3K WG (BYDV) Flexi® Vectors.

Features:

• Save Time: Generate proteins in a few hours rather than days.
  
   
• Versatility to Suit Your Needs: You can label, purify or use protein directly for many applications.
  
   
• Increased Solubility: No need to worry about solubility issues associated with E. coli systems.
  
   
• Perform Expanded Applications: Wheat Germ Extract Plus can generate up to 20-fold more protein (80μg/ml) than other standard wheat germ systems.
  
   
• No Need to Purchase Expensive Instrumentation: Use in batch format.

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