The TNT® Coupled Wheat Germ Extract Systems offer researchers an alternative for eukaryotic cell-free protein expression: a one-tube, coupled transcription/translation system. The TNT® Extract Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TNT® Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TNT® Extract reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates. Magnesium Acetate, 25mM, and Potassium Chloride, 2.5M, can be used to optimize in vitro translation reactions in the TNT® T7 Quick Coupled Transcription/ Translation System, Flexi® Rabbit Reticulocyte Lysate System and TNT® Coupled Wheat Germ Extract System.

Features:

• Reliable: The TNT® Systems are rigorously quality controlled to ensure the highest level of transcription/translation, whether your template is a linear (T3 or T7 only) or circular plasmid.
• Convenient: Single-tube procedure eliminates the time and effort required to prepare RNA for a standard wheat germ translation. Translation results can be visualized by autoradiography in 6–8 hours.
• Versatile: The T7 and T3 systems will produce protein from both linear and circular DNA (use only circular DNA with the SP6 system; for PCR templates use Cat.# L5540, TNT® T7 Quick for PCR DNA).
• Controls Included: Luciferase Control DNA and Luciferase Assay Reagents are included with the system as functional controls. Only full-length luciferase is active.

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