High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo^(R) Luciferase Assay System is designed for this purpose by providing long-lived luminescene when added to cultured commonly used cell culture media without prior sample processing. Throughout rates of several thousand samples per hour may be achieved with high reproductibility under standard laboratory conditions.

Features:

• Greater Light Output: Results in greater assay sensitivity than other leading extended-lifetime firefly luciferase assay reagents.
  
   
• Improved Assay Precision: Less sensitive to mixing conditiond for improved assay reproducibility in multiwell plates. This is particularly useful in 384-well plates.
  
   
• Convinient: Simply mix buffer with lyophilized substrate and use; there is no need for thawing or measuring before use.
  
   
• Quick: No need to remove culture medium or wash cells prior to adding assay reagent. Cells may be grown and assayed directly within the same multiwell plate.
  
   
• Easy to Use: Simply add reagent, which contains a cell lysis component, wait 5 minutes and measure luminescene.
  
   
• Robust: Compatible with many tissue culture media, including those containing up to 10% serum.
  
   
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway

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