The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, upto 60 mg of tissue can be processed per purification, depending on the tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. this step sustantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation.

Features:

• Safety and Efficiency: Rapid isolation of high yields of total RNA without the use of hazardous compounds like phenol.
• Flexibility: Single system for isolation directly from blood, cells or tissues. Two methods avialble for purification: microcentrifugation (spin) or vacuum.
• Confidence: Purified RNA suitable for routine molecualr biology applications, including RT-PCR and Northern Blotting.

Back