The PolyATtract(R) mRNA Isolation Systems use the MagneSphere(R) technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3' poly(A)+ regiobn present in most mature eukaryotic mRNA's. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from solid phase by the simple addition of ribonuclease-free, deionized water. with total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications including, in vitro translation and cDNA synthesis.
Improved Productivity: Entire mRNA purification process can be completed in approximately 45 minutes.
Highly Pure mRNA: Due to the strength and selectivity of the interraction between streptavidin and biotin, mRNA bound to the biotinylated oligo(dT) is captured by the streptavidin-coated magnetic particles.
Confidence in Your Applications: Isolated mRNA is suitable for use with in vitro translation, RT-PCR and cDNA synthesis.
Flexibility: Configurations for use with small or large amounts of cells and tissues.