ImProm-II^(TM) Reverse Transcription System produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components of ImProm-II^(TM) Reverse Transcription System can be used to reverse transcribe RNA templates starting with total RNA , poly(A)+mRNA or synthetic transcript RNA. The optimized reaction buffer and powerful ImProm-II^(TM) Reverse Transcriptase provided in the ImProm-II^(TM) Reverse Transcription System together enable robust, full-length, cDNA syntheis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions have been formulated for stand alone applications or for easy transition to gene-specific target amplification. From 1-20u l of the reverse transcription reaction can be directly amplified using Taq DNA polymerase in coupled or uncoupled PCR.

Features:

• Full Length:
  reverse transcribe long RNA template up to 8.9kb
• Microarray-Compatible:
  May be used for incorporation of regular, Cy(R)3-modified, Cy(R)-5 modified and amino-allyl-modified nucleotides.
• Easy to Use:
  Kit format provides all reagents necessary for efficient reverse transcription.
• Scalable and Flexible:
  From 1-20μl from the initial RT reaction may be used in subsequent PCR, and the optimized buffer also akkows coupled RT-PCR.
• Versatile:
  Use with your thermostable DNA polymerase of choice. 

Back