Flexi^(R) Vector System is a simple, yet powerful, directional cloning method for protien-coding sequences. It is based on two rare-cutting restriction enzymes, Sgfi and Pmel, and provides a rapid, efficient and high-fidelity way to transfer protien-coding regions between a variety of Flexi^(R) Vectors without the need to resequence.

All Flexi^(R) Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as positive selection for the successful ligitation of the insert.

Unlike site-specific recombination vector systems, the Flexi^(R) Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of the protien of interest. in addition, the systems do not require an archival entry vector, and most applications allow direct entry into the vector suited to the experimental design.

C-Terminal Flexi^(R) Vectors allow expression of C-Terminal-tagged protiens. While these vectors can act as acceptors of protien-coding regions flanked by Sgfl and Pmel, they lack a Pmel site and contain a different blunt-endfed site, EcoIRCI. This joined sequence cannot be removed from the C-terminal Flexi^(R) Vectors and transferred to other Flexi^(R) Vectors.

Features:

• Versatility: You can choose between a variety of initial applications (e.g., bacterial protien, mammalian, or cell-free protien expression) and then transfer to others as required.
  
   
• Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies.
  
   
• Enhanced Productivity: adaptable to high-troughput formats for large screening projects
  
   
• Easy Access: No licensing fees or complicated transfer restrictions.

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