Dual-Glo^(R) Luciferase Assay System is a homogenous reagent system that enables fast and simple quantitation of a stable luminiscent signal from two reporter genes in a single sample. This convinient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo^(R) Luciferase Assay System provides high Z-factors for cell-based, high-throughput screening applications. with the Dual-Glo^(R) System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings caused by non specific factors such as cytotoxicity. In the Dual-Glo^(R) Luciferase Assay, the activity of the co-transfected controlreporter provides an internal control to normalize things. The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. 

Features:

• Increased Precision and Accuracy: Normalize primary reporter results with an internal control, a co-reporter that minimizes effects of cell number and health, transfection efficiency and non specific cellular responses.
  
  
  
• Homogeneous Format: Perform fewer steps. Assay cells directly in growth medium for both reporters. No centrifugation or lysis step is required.
  
   
• Stable Signal: Obtain flexibility for either batch or continous processing of 96- and 384-well plates. Each lumniscent signal may be measured for up to 2 hours after reagent addition.
  
   
• Convinience: Screen efficiently with this simple, two-step assay that is ideal for any luminometer. On-board injectors not required.
  
   
• Wide Dynamic Range: Analyze high and low reporter activity without sample diliution. Linear over at least 6 logs of enzyme concentration for each reporter.
  
   
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway

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