The AccesQuick(TM) RT-PCR System is an easy and convinient master mix system for setting up one-tube RT-PCR. It is designed to increase convinience by combining the following components in single tube: TfI DNA Polymerase, dNTPs, magnesium sulfate and reaction buffer. The AMV RT enzyme is provide in a separate tube to allow important, no-RT control reactions. The AccessQuick(TM) Master Mix is simply added to RNA templates in reaction vials, followed by AMV RT, primers and water. The AccesQuick(TM) RT-PCR Master Mix is intended for routine RT-PCR applications that have been previously optimized and do not require extreme conditions.

Features:

• Achieve Maximum Convinience: Save yourself from four pipetting steps. Simply combine AccesQuick(TM) RT-PCR Master Mix, AMV RT, your gene-specific primers, your RNA template and water. Separate AMV RT allows important, no-RT control reactions.
   
• Use Less Template: Amplify from zeptomole (10(-21)mol) levels of RNA.
   
• No Buffer Additions Required: Set up reactions in asingle tube, drop into thermal cycler, come back later for results-no additions between the reverse transcription and DNA amplification.
   
• Stable: System components are stable over many freeze-thaw cycles.

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